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1994-10-25
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Document 2712
DOCN M94A2712
TI Evaluation of HIV-1/HIV-2 antibody tests in developing countries.
DT 9412
AU Maskill W; Belsey E; Tamashiro H; Gershy-Damet G; Liomba G; M'Boup S;
Wasi C; Fairfield Hospital, Melbourne.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):241 (abstract no. PB0392). Unique
Identifier : AIDSLINE ICA10/94369863
AB OBJECTIVE: To assess the sensitivity and specificity of HIV-1/HIV-2
ELISA and Simple/Rapid (S/R) assay under field conditions. METHODS:
Eight enzyme-linked immunosorbent assays (ELISA's) and five simple/rapid
(S/R) tests were evaluated under field conditions in four countries,
Cote d'Ivoire, Malawi, Senegal and Thailand. Approximately 400 routine
serum samples were tested on three S/R assays at each of five peripheral
laboratories in each country. All serum samples were retested by at
least three ELISA's at a central laboratory. Samples reactive by an
ELISA or S/R assay underwent Western Blotting. Samples found to be
Western Blot positive according to WHO criteria were considered HIV
antibody positive. Sera which were either negative by all S/R assays and
ELISA's or which were Western Blot negative were considered HIV antibody
negative. The sensitivity, specificity and delta values were calculated
for each test. RESULTS: Sensitivity estimates for S/R tests ranged from
86.6% to 99.4% and 80.4% to 100% for ELISA's. Specificity varied from
96.4% to 100% for S/R assays and 86.9% to 100% for ELISA's. Variation in
sensitivity and specificity estimates appeared to be both test and
country dependant and could not be solely attributed to laboratory
technique. The delta analysis assisted in assessing these variations.
DISCUSSION AND CONCLUSION: The sensitivity and specificity of
HIV-1/HIV-2, ELISA's and S/R tests preformed in field laboratories can
in some cases approximate that achieved by reference laboratories.
Efforts should be made to identify and correct factors responsible for
reducing test performance.
DE *AIDS Serodiagnosis Comparative Study Cote d'Ivoire *Developing
Countries Enzyme-Linked Immunosorbent Assay Human HIV
Antibodies/*BLOOD HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY Malawi Predictive Value of Tests
Quality Control Senegal Thailand MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).